Study identifies newgene that drives coloncancer mountsinainyc NatureComms
was acquired from NCBI and PCR amplified from HT29 cell cDNA, derived as above. Inserts were cloned into the doxycycline-inducible pTRI-blas vector, a gift from Dr. Poulikos Poulikakos and Dr. Stuart Aaronson using the EcoR1 and BamH1 restriction enzyme sites . Thecarboxy-terminus comprises a PDZ binding domain. To minimize disruption of this domain which may require a free carboxy-terminus, the V5 peptide tag was inserted in-frame immediately upstream of the PDZ binding domain.
using default parameters. Principal component analysis was performed using deepTools multiBigwigSummary and plotPCA functions. Summary plots were generated using deepTools computeMatrix and plotProfile. Pearson correlation heatmap and unsupervised hierarchical clustering analysis was performed using deepTools plotCorrelation.
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