Active targeting and cytosolic delivery of therapeutic payloads are challenging in small extracellular vesicle-based drug delivery systems. Here, the authors engineer fusogen and targeting moiety co-functionalized cell-derived nanovesicles, which can selectively bind to target cells and efficiently fulfill cytosolic delivery through membrane fusion.
. In brief, the existing engineered EVs did not fully demonstrate the capability of cancer-targeting cytosolic drug delivery. The membrane fusion efficiency was also modest. These shortcomings, on the contrary, reflect the strengths of our eFT-CNVs. One more point to emphasize is that the mechanical extrusion of donor cells can generate a large number of nanovesicles in only a few minutes, and the average batch-to-batch variation can be well controlled with optimal manufacturing parameters.
Our study demonstrated that drug-loaded nanovesicles achieved enhanced treatment efficacy compared to free medications. Moreover, compared to nanovesicles without targeting moieties or fusogens, eFT-CNVs can further improve treatment efficacy by delivering drugs to cytosol directly. The effect was particularly obvious in the delivery of gelonin. Gelonin lacks carbohydrate-binding domains, and thus it cannot penetrate cell plasma membranes, making it ineffective in cell treatments.
In addition to drug delivery, the developed eFT-CNVs could be used in cell editing and vaccine applications. For example, conferring specific neoantigens to immune cold tumor cell surface could transform them into immune hot ones, encouraging immune cell-mediated tumor killing. The targeted cytosolic delivery of CRISPR/Cas9 complex with eFT-CNVs would enable specific and efficient gene editing.
Australia Latest News, Australia Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
Cultural-ecosystem resilience is vital yet under-considered in coastal restoration - Humanities and Social Sciences CommunicationsHumanities and Social Sciences Communications - Cultural-ecosystem resilience is vital yet under-considered in coastal restoration
Read more »
China puts its flexible solar paneled communications satellite into orbitThe 1 mm thick solar panel folds in less than two inches of thickness but stretches to 30 feet in length and is eight feet wide when unfolded.
Read more »
Dogecoin (DOGE) Whales Are Active, Here's How Much They Moved WTDICYMI: according to IntoTheBlock, the total whale accumulation of Dogecoin jumped significantly in the week-to-date period DOGE $DOGE DOGEArmy
Read more »
Jeff Bezos playing increasingly active role in WaPo's operations: NYTAmazon founder Jeff Bezos is playing an increasingly active role in The Washington Post's operations, report says
Read more »
Ancient Underwater Volcano Off Canada Is Still Active, And Teeming With LifeA submerged volcano is exuding hot fluid deep below the sea off the Pacific coast of Canada, and thousands of eggs look like ravioli on its summit.
Read more »