Candidate hepatitis C mRNA vaccine induces protective immunity in animal model AnimalModel Hepatitis HepatitisC Immunity Vaccine Nature SLU_Official Penn
Study: Hepatitis C virus E1 and modified E2 delivered from an mRNA vaccine induces protective immunity. Image Credit: Kateryna Kon / Shutterstock
About the study In the present study, researchers evaluated the immunogenicity of the HCV ectodomains of E1 protein in mice who were and were not vaccinated with the soluble E2 mRNA-lipid nanoparticles vaccine. HCV-lentiviral pseudotype particles reflecting homologous H77C neutralization by sera from individually vaccinated animals were examined to determine the relative antibody neutralization associated with each HCV envelope-mRNA candidate vaccine. Using enzyme-linked immunosorbent assay , the researchers also examined Th1 and Th2 specific cytokines in the serum samples of mice vaccinated with the HCV envelope-mRNA-LNP-vaccine.
Furthermore, in the presence of native HCV E2 mRNA-LNPs, the protective cellular response induced by the administration of mRNA-LNPs expressing HCV E1 was significantly diminished, demonstrating that HCV E2 may have a modifying influence on co-inoculated immunogens. Th1-specific cytokines, namely IL-2 and IFN-γ, were considerably raised in the sE2F442NYT-immunized cohort, but the sE1-immunized group also exhibited elevated levels of IL-2 and IFN-γ. Mice inoculated with sE1/sE2 combined or only sE2 had low levels of Th1-specific cytokines. In contrast, the Th2-specific cytokines, namely IL-4 and IL-10, were elevated in vaccinated mice exposed to sE1/sE2 in combination or only sE2.
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