A study published in BMCMicrobiol finds that animal-origin Staphylococcus pseudintermedius may play a role in the development of atopic dermatitis, emphasizing the importance of taking preventive measures when in contact with animals.
This study has been reviewed and approved by the Ethics Committee of Renji Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China . The legal guardian of the young patient provided informed consent.
The PMseq infection pathogen high-throughput gene detection kit was used for end repair, adapter ligation, and PCR amplification to establish a DNA library. The Qubit dsDNA HS Assay kit was used to detect the library concentration and prepare DNB . The ssDNA detection kit was used to detect DNB concentration .
The MGISEQ-2000 gene sequencer manufactured by MGIU was used for advanced sequencing with the Single-End 50 bp sequencing mode. More than 80 M reads sequencing data volume were acquired to make sure that the sequencing data is enough for the following analysis. The low-complexity sequences, repetitive sequences, low-quality sequences, and human sequences were filtered in sequencing data by MGISEQ-2000 gene sequencer. For bioinformatic analysis, the obtained high-quality sequencing sequences were compared with multiple pathogen reference genome source databases to annotate the species of pathogens.After serial dilution of the lip swabs, the samples were spread on the sheep blood agar plate and cultured at 37°C for 24 h aerobically.
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Associations between dietary patterns and an array of inflammation biomarkers and plasma lipid profile in postmenopausal women - BMC Women's HealthObjective and design In this cross-sectional study, evaluation of the association between four dietary patterns, nutrients and food intakes and an array of systemic inflammation biomarkers and lipid profile among 80 New Zealand postmenopausal women were conducted. Materials Eighty postmenopausal women participated in the study. A validated food frequency questionnaire was used to collect nutrients and food intake. Four dietary patterns were identified by principal component analysis (PCA) and plasma samples collected for inflammatory biomarkers and lipid profile measures. Results There were negative correlations between intake of dietary fibre, soluble and insoluble non-starch polysaccharides (NSP), vitamin C and niacin and with almost all the inflammatory markers for the whole group. Vegetables, tea/coffee and especially fruit intake were negatively correlated with the inflammatory biomarkers in the whole group. A high intake of Pattern 1 (potato, bread, and fruit pattern) was associated with a low risk of high interferon (IFN)-α2, IFN-λ, interleukin (IL)-6 and IL-8 levels while a high intake of Pattern 3 (fast-food pattern) was associated high risk of IFN-α2 levels. Multiple linear regression showed a negative correlation between Pattern 2 (soups and vegetables pattern) and levels of C-reactive protein (CRP) as well as ferritin. A positive association was observed between Pattern 3 (fast-food pattern) and CRP levels. Positive correlation was also observed between Pattern 2 and high-density lipoprotein (HDL) and total cholesterol (TC) levels, Pattern 4 (meat and vegetables pattern) was however negatively correlated with TC, low-density lipoprotein (LDL) and TC/HDL ratio. Conclusions The result of this study reinforces the contribution and role of diet in modifying inflammation in postmenopausal women.
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The role of community healthcare professionals in discussing sexual assault experiences during obstetrics and gynecological healthcare appointments - BMC Women's HealthBackground Nearly half of adult women in the US report experiencing sexual assault, with almost one-fifth reporting rape. For many sexual assault survivors, healthcare professionals are the first point of contact and disclosure. This study aimed to understand how healthcare professionals working in community settings perceived their role in discussing sexual violence experiences with women during obstetrical and gynecological healthcare appointments. The secondary purpose was to compare healthcare professionals’ perspectives with the patients’ to determine how sexual violence conversations should occur in these environments. Methods Data were collected in two phases. Phase 1 consisted of 6 focus groups (Sept-Dec, 2019) with women aged 18–45 (n = 22) living in Indiana who sought community-based or private healthcare for women’s reproductive healthcare needs. Phase 2 included 20 key-informant interviews with non-physician healthcare professionals (i.e., NP, RN, CNM, doula, pharmacist, chiropractor) living in Indiana (September 2019-May 2020) who provided community-based women’s reproductive healthcare. Focus groups and interviews were audio-recorded, transcribed, and analyzed using thematic analyses. HyperRESEARCH assisted in data management and organization. Results There were three resulting themes: (1) healthcare professionals’ approaches to screening for a history of sexual violence varied depending on how they ask, what setting they work in, and type of professional asking; (2) healthcare experiences can compound traumatic experiences and create distrust with survivors; and (3) sexual violence impacts patient healthcare experiences through what services they seek, how professionals may interact with them, and what professionals they are willing to utilize. Conclusions Findings offered insight into actionable and practical strategies for enhancing sexual violence screening and discussions in community-based women’s reproductive health settings. The findings offer
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