A novel deep brain simulation approach for treating drug-refractory epilepsy NatureComms
; L1, L2, L3, L4, R4, R3, R2, and R1, in that order; a 100 µs interval between each pulse). Stimulation was continuously applied until the seizure was terminated, and a non-stimulation interval of 10 s was set for each stimulus to prevent excessive stimulation. Electrical artifacts could be recorded and disrupt the computation for seizure detection during stimulation, thus the seizure detector was turned off when stimulation was in progress.
Following the chronic seizure induction and electrode implantation procedures, animals were monitored 24 h a day to detect electrographic seizures with the algorithm that uses combinations of frequency and spike features. When the specified threshold for intervention was met, SNF or LIWF stimulation was immediately applied to the hippocampal region in each stimulated group . Stimulation was applied only once for each individual event, and a non-stimulation interval was given as 100 s.
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