A study published in Cancer Cell International finds that small extracellular vesicles-educated hepatocytes in colorectal cancer may have an active role in the early stage of liver metastasis formation.
] with minor modifications. The tissue pieces were gently sliced into small fragments and incubated with collagenase D and DNase I dissolved in RPMI plain medium for 30 min at 37 °C. After incubation, the samples were passed through a 70 µm filter. The resulting filtered liquid was centrifuged at 300 × g for 10 min and 2,000 × g for 20 min and ultracentrifuged at 16,500 × g for 6 min to remove cells, tissue debris, and large EVs.
1. For sEVs isolated from conditioned culture medium and plasma samples, sEVs were prepared for electron microscopy studies using negative staining. Approximately 5 μl of the sEV suspension was deposited onto carbon-coated EM grids. After washing, the samples were fixed for 5 min in 1% glutaraldehyde and negatively stained with a 2% aqueous solution of phosphotungstic acid. The grids were viewed in a JEM 1400 Plus electron microscope operating at 80 kV equipped with a CCD camera.
Australia Latest News, Australia Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
Eating garlic may lower risk of colorectal cancer, study findsResearch investigates the association between garlic consumption and colorectal cancer risk, as well as the influence of garlic consumption on bacterial DNA in the blood. The study found that garlic consumption was inversely associated with colorectal cancer risk and that it influenced the gut microbiota.
Read more »
Multiple sclerosis plasma IgG aggregates induce complement-dependent neuronal apoptosis - Cell Death & DiseaseCell Death & Disease - Multiple sclerosis plasma IgG aggregates induce complement-dependent neuronal apoptosis
Read more »
JCI - IL-18-secreting CAR T cells targeting DLL3 are highly effective in small cell lung cancer models
Read more »
The transcriptional and regulatory identity of erythropoietin producing cells - Nature MedicineSingle-cell analyses in a reporter mouse model and human tissues identify a rare cell subset that produces erythropoietin in vivo, opening potential new avenues for research in erythropoiesis and oxygen homeostasis.
Read more »