It's believed to be one of the worst road accidents on record in the North African nation.
The bus had left the capital, Tunis, that morning with 43 people on board and was headed to the northern mountain town of Ain Draham, a popular destination for Tunisian tourists, according to a statement from Tunisia's Ministry of Interior.
In addition to those who died, another 18 people were injured and rushed to local hospitals, according to a statement from Tunisia's Ministry of Public Health.
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