Anti-inflammatorydrugs commonly taken by children can cause alterations to dental enamel, study shows SciReports
was used for standardization and calibration of measurements. The mean gray-value of the mineral grains were set to as the electron densities of 3.17 g/cm. The volume, thickness and density of enamel was measured from the CT-scans central section of the incisors at the point just in front of the margin of the lower jaw bone.The jaws were dissected and removed with surgical scissors.
Next, slides were washed and incubated with mouse anti-goat and mouse anti-rabbit biotinylated secondary antibody for 1 h, washed in PBS, and incubated with streptavidin conjugated with horseradish peroxidase for 20 min. 3,3′-diaminobenzidine was used as the enzyme substrate for 5 min; the slides were washed with PBS, counterstained with hematoxylin for 15 s, washed with distilled water, dehydrated in increasing ethanol concentrations, and mounted in Entellan.
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