Researchers develop new method for specializing and purifying human stemcells into interneurons kingscollegelon SciReports
i), suggesting that the full conversion and maturation in human cells could take longer, even in the presence of neuronal maturation-stimulating growth factors [brain-derived neurotrophic factor and neurotrophin-3 ].In this study, we report the successful enrichment of human PSC-derived V3 spinal interneurons and their progenitor population.
–based purification—a scalable and a cost-effective system—and we were able to recover highly enriched populations of CD14V3 and the OLIG2MN progenitor marker expression; the transcriptional separation of these two lineage markers was investigated and determined to occur in later stages of neurogenesis. Therefore, when usingbased
sorting, we found that sorting at later timepoints successfully reduces MN-associated markers, such as ISL1, from the culture. -based sorting enriched V3 progenitors from ~ 40% in mixed differentiation cultures to > 90% in the eluate fraction. Post-mitotic conversion and functional maturation of the human PSC-derived neurons is still a challenge within the field, partly due to limited research on their survival requirements, as well as slow development in a human model
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