Assessing the risk of excess folic acid intake BCMHouston
. To this end, we sought to quantify the potential impact of FA supplementation on de novo spontaneous mutation rates and its effect on whole-genome methylation modification.
C57BL/6 J mice were maintained on three different FA diets, including FA-low , FA-control , and FA-high for four months. According to the conversion of animal doses to human equivalent doses based on body surface area used in the “US FDA 2005 Guideline for Industry”, 3 ppm equivalent to an average of 0.11–0.18 mg per day for an adult mouse which eats 3–5 g of mouse chow per day, about a quarter to a half of the US CDC RDA . The 30 ppm FA mouse diet is the equivalent up to 1.
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