Nature research paper: Fast and efficient DNA replication with purified human proteins
, Standard replication reaction on the 9.7 kbp template performed with the indicated proteins and analysed by native and denaturing agarose gel electrophoresis as indicated. In the absence of RFC and PCNA the predominant replication products migrate above the position of full length in the native gel. As indicated, in the native gel template labelling products and complete full-length replication products migrate in the same position.
, Denaturing agarose gel analysis of a pulse chase experiment on the 9.7 kbp template with the indicated proteins., Denaturing agarose gel analysis of a replication reaction on the 9.7 kbp template with the indicated proteins., Denaturing agarose gel analysis of a pulse chase experiment on the 9.7 kbp template with the indicated proteins., Silver-stained SDS-PAGE analysis of glycerol gradients performed with the indicated proteins.
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