How heartfailure leads to cognitive decline ColumbiaMed NatureNeuro
. fEPSPs were evaluated at the Schaffer collateral by a bipolar electrode placed at the CA3 and recording at the CA1 with an artificial CSF-filled glass pipette. Basal synaptic transmission was evaluated via measurement of the I–O curve via plotting the relationship between increased voltages and evoked fEPSP responses. A 30-min baseline was recorded every minute at a voltage eliciting 35% of the maximum evoked response as determined by the I–O curve.
Proteins were transferred onto nitrocellulose membranes for 1 h at 200 mA. Immunoblots were developed using the following primary antibodies : anti-RyR2, anti-phospho-RyR-Ser-2808, anti-Cys-NO, anti-calstabin and anti-NOX2. The channel’s oxidation was determined by probing the carbonyl groups in the protein side chains by reaction with 2,4-dinitrophenylhydrazine.
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