The Integrated Portable Droplets system (iPODs) device offers a portable, affordable, and user-friendly droplet analysis system for rapid-results testing with strong accuracy in detecting and quantifying bacterial species. Rapid-results testing has become an increasingly important part of our fas
value is to 1, the better the line is able to visually represent how well the iPODs device can detect and quantify the bacterial species present in the sample .
“In previous reports, droplet reinjection undoubtedly requires precise fluid control, thereby reducing the portability of the device, more seriously, restricting the development of droplet-based nucleicamplification testing for POCT,” said LIU Fengyi, a doctoral student and first author of the study. “Here we showed this droplet reinjection chip greatly simplified the experimental setup and operation procedure with low device cost and low reagent consumption.
Droplet digital LAMP with iPODs makes it highly sensitive and accurate in assessing quantities of nucleic acids present in the sample. The downside to this is the need for precise fluid control and external pumps, all of which reduce portability and increase costs.
In order for this technology to reach its full potential, some adjustments will be needed in the future, such as improving the stability of the system, and implanting of thermal cycling modules for droplet digital PCR . Further implantation with other cheap chip materials for large standardized production, as well as the introduction of fully automated operation processes, will make the system more user-friendly.
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