US Democratic presidential nominee Joe Biden speaks with Jacob Blake, who was shot seven times by police, and meets with his family in Wisconsin after more anti-police protests.
Mr Trump has threatened to defund city governments that are experiencing protests over police brutality.
But the US Conference of Mayors said the order had no legal standing, and would make cities less safe by slashing resources for police and first responders. "Trump needs to wake up to the reality facing our cities — and our entire country — and realise he is not above the law," the mayors said in a joint statement.
The United States is experiencing escalating clashes between right-wing and left-wing groups as police shootings of black people spark protests and counter-protests in the run-up to the November 3 presidential election.
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