A neuronal network that represents the direction in which zebrafish are traveling NatureNeuro
). The chamber was filled with water from the fish facility system and agarose was removed along the optic path of the lateral laser beam and around the tail of the animal . In some larvae, the eyes were also freed from the agarose. After embedding, fish were left recovering for 1–6 hours before the imaging session. Before starting the imaging, we lightly tapped on the side of the chamber to select the most active fish for the experiment.
The stimulus presentation and behavior tracking were synchronized with imaging acquisition using a ZeroMQ-based trigger signal supported natively by Stytra.For two-photon experiments, animals were embedded in 2% low-melting-point agarose in 30-mm petri dishes. The agarose around the tail, caudal to the pectoral fins, was cut away with a fine scalpel to allow for tail movement.
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