A study in Environmental Microbiome highlights an unexpected level of compositional heterogeneity across fractured shale formations and the ability of diverse microbial community to tolerate the extreme conditions of the deep subsurface.
]. In contrast, due to restricted access, the 8 samples from Bowland-2 were taken from water storage tanks several weeks after flow back. Well operators collected the samples in sterile 500 ml Nalgene bottles which were filled to capacity to minimize headspace. Samples were stored at 4 °C on site until they were transported to the University of Manchester. In the laboratories, the samples were aliquoted for DNA sequencing and geochemical analyses.
pH was measured with Denver Instrument digital meter and Fisherbrand FB68801 electrode.The biomass in the samples was concentrated by centrifugation at 4000 rpm in a BOECO C-28A centrifuge for 15 min. DNA from Bowland-1 flowback fluids was extracted from the concentrated samples with the DNeasy PowerSoil Pro Kit . Bowland-2 concentrated samples were passed through a sterile 0.2 μm filter using vacuum filtration and the DNA was extracted from filters with a Dneasy PowerWater Kit .
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