Opening doors for broader participation in oral microbiome studies: the stability and comparability of ethanol-free mouthwash PLOSONE microbiome oralmicrobiome ethanol ethanolfree alcoholfree mouthwash study research
By Pooja Toshniwal PahariaMay 1 2023Reviewed by Lily Ramsey, LLM In a recent study published in PLOS ONE, researchers compared the use of ethanol-free mouthwash and ethanol-containing mouthwash for oral microbiome studies and assessed the stability of the mouthwash samples for ≤10 days before processing.
Individuals might refrain from using ethanol-containing mouthwashes because of the associated burning-type sensation and/or other medical, personal, cultural, and/or religious concerns. The two samples were obtained on consecutive days, wherein 50% of the participants provided ethanol-free mouthwash samples on the initial day and ethanol-containing mouthwash samples on a subsequent day, and vice versa for the remaining half of the study participants. At the laboratory, three aliquots of 3.0 mL each were created from all samples.
In addition, the included individuals had a full set of teeth, worked at the Frederick National Laboratory or were members of the Fort Detrick community, and weighed ≥110.0 pounds. The ethanol-free mouthwash comprised cetylpyridinium chloride , glycerin, water, flavor, sodium saccharin, poloxamer 407, sucralose, methylparaben, Blue 1, and propylparaben.
High ICCs, above 0.75, were observed for the most abundant genera and phyla , concerning the comparability of the two types of mouthwashes.
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