Environmental toxin PCB found in deepsea trench UniSouthDenmark NatureComms
Geochemical measurement of the sediment organic carbon was conducted in the Lithospheric Organic Carbon lab, Department of Geoscience, Aarhus University. The method involves programmed temperature, open anhydrous pyrolysis, and combustion of ~50 mg dry bulk sediments using the HAWK Pyrolysis and TOC analyzers . Concentrations of the labile protohydrocarbons were measured using a FID detector at iso-temperature of 300 °C.
Due to the low expected concentrations, a large volume injection method was used. In brief, 10 µL sample were injected into a programmable temperature vaporizing inlet, where the excess solvent was evaporated at low temperatures prior to injection onto the column. Each congener was analyzed with a minimum of two transitions in the MS/MS detector . The data was quantified based on the transition ion with the strongest signal of each analyte, using an internal standard method.
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