Study highlights progesterone's protective potential against Parkinson's ruhrunibochum
], 10 μL GoTaq qPCR Master Mix , 1.4 μL primer upstream, 1.4 μL primer downstream, diluted cDNA and ddH2O were combined to a final volume of 20 μL. Using the CFX96 Real Time PCR Detection System samples were heated to 95 °C for 2 min, followed by 40 amplification cycles, 15 s at 95 °C and 60 s at 60 °C.Immunofluorescence staining and confocal laser scanning microscopy at 40× of the myenteric ganglia was performed using the protocol described in Hecking et al. [].
The myenteric plexus cells of male Wistar rats were cultivated after several steps of preparation and enzymatic digestion as previously described in Hecking et al. []. The preparation tools and Petri dishes were sterilized at 240 °C for 4 h, and the work surfaces were cleaned with NaOH-EDTA beforehand. All solutions were prepared under sterile conditions at an appropriate workbench to avoid contamination. They were sterilized by membrane filtration or autoclaving.
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Prevalence and risk factors of significant persistent pain symptoms after critical care illness: a prospective multicentric study - Critical CareBackground Prevalence, risk factors and medical management of persistent pain symptoms after critical care illness have not been thoroughly investigated. Methods We performed a prospective multicentric study in patients with an intensive care unit (ICU) length of stay ≥ 48 h. The primary outcome was the prevalence of significant persistent pain, defined as a numeric rating scale (NRS) ≥ 3, 3 months after admission. Secondary outcomes were the prevalence of symptoms compatible with neuropathic pain (ID-pain score | 3) and the risk factors of persistent pain. Results Eight hundred fourteen patients were included over a 10-month period in 26 centers. Patients had a mean age of 57 (± 17) years with a SAPS 2 score of 32 (± 16) (mean ± SD). The median ICU length of stay was 6 [4–12] days (median [interquartile]). At 3 months, the median intensity of pain symptoms was 2 [1–5] in the entire population, and 388 (47.7%) patients had significant pain. In this group, 34 (8.7%) patients had symptoms compatible with neuropathic pain. Female (Odds Ratio 1.5 95% CI [1.1–2.1]), prior use of anti-depressive agents (OR 2.2 95% CI [1.3–4]), prone positioning (OR 3 95% CI [1.4–6.4]) and the presence of pain symptoms on ICU discharge (NRS ≥ 3) (OR 2.4 95% CI [1.7–3.4]) were risk factors of persistent pain. Compared with sepsis, patients admitted for trauma (non neuro) (OR 3.5 95% CI [2.1–6]) were particularly at risk of persistent pain. Only 35 (11.3%) patients had specialist pain management by 3 months. Conclusions Persistent pain symptoms were frequent in critical illness survivors and specialized management remained infrequent. Innovative approaches must be developed in the ICU to minimize the consequences of pain. Trial registration. NCT04817696. Registered March 26, 2021.
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Trapped wind in pregnancy: how to relieve it and why it happensTrapped wind can cause stomach pains and cramping in early pregnancy, but there are a few ways you can ease it. 💨
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Pharmacies told to limit supplies of popular HRT treatment for patients due to shortageMinisters have limited the supply of one of the most popular hormone replacement therapies to try and cope with rising demand 🔴 The Government said the move will allow pharmacists to dispense a maximum of two months’ supply per prescription
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