Q-LAAD: a dynamic, cost-effective rapid antigen test for detection of SARS-CoV-2 medrxivpreprint BoiseState SARSCoV2 COVID19 coronavirus covid
By Dr. Chinta SidharthanSep 28 2022Reviewed by Danielle Ellis, B.Sc. In a recent study posted to the medRxiv* preprint server, researchers developed a high-throughput, rapid antigen severe acute respiratory syndrome coronavirus 2 spike protein detection test using allonamers, a new type of aptamers that carry out allosteric regulation instead of catalytic activity.
Antigen-detecting rapid diagnostic tests target viral spike and nucleocapsid proteins in the respiratory tract and are a more suitable option for early detection of COVID-19. However, their dependence on highly specific monoclonal antibodies limits their widespread usage. A DNA allonamer was produced using the systematic evolution of ligands by exponential enrichment . This allonamer consisted of two binding domains with a linker region. The binding of the SARS-CoV-2 spike protein to one domain would result in a conformational change in the other binding domain, allowing it to connect to a fluorescent reporter molecule.
When Q-LAAD was tested against the alpha, beta, gamma, and delta SARS-CoV-2 variants, the fluorescence signals from detecting gamma and delta spike proteins were significantly higher than the nucleocapsid control, while the signals from binding to alpha and beta spike proteins showed a five-fold change, although it was not significant.
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