A research team led by Prof. Gong Lei from the University of Science and Technology (USTC) of the Chinese Academy of Sciences (CAS) and collaborators developed a three-dimensional single-pixel imaging (3D-SPI) approach based on 3D light-field illumination(3D-LFI), which enables volumetric imaging of microscopic objects with a near-diffraction-limit 3D optical resolution.
They further demonstrated its capability of 3D visualization of label-free optical absorption contrast by imaging single algal
in vivo. The study titled"Optical Single-Pixel Volumetric Imaging by Three-dimensional Light-Field Illumination" was published inSPI has become an attractive 3D imaging modality. Through single-pixel detectors instead of conventional array sensors, the performance of SPI exceeds the conventional ones in spectral range, detection efficiency, and timing response.
To exceed the resolution limitation, the researchers built a 3D-LFI-SPM prototype. As a result, the prototype achieves an imaging volume of ~390×390×3,800 μmand a resolution of up to 2.7 μm laterally and 37 μm axially. They performed label-free 3D imaging of living Haematococcus pluvialis cells and successfully counted thePredictably, the approach can be applied to visualize various absorption contrasts of biological samples.
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