Nature research paper: RNA polymerase II associates with active genes during DNA replication
, Cells were labelled for 5 min with EdU, chased for the indicated times, and then CAA was performed for PCNA. Data are presented as mean values ± s.d. , and 208 nuclei were examined over 3 independent experiments. Statistical significance was determined by one-way ANOVA and p-values shown were determined with Tukey’s post-hoc. ns=not significant, ****, Sequential ChIP with PCNA and SPT4.
. Lower panel: re-ChIP of samples first immunoprecipitated for SPT4. Following elution, samples were re-immunoprecipitated for PCNA or IgG. Following DNA purification, samples were analyzed by qPCR. Data are presented as mean values and are representative of 2 independent experiments., Western blotting validation of PCNA and FEN1 polyclonal antibodies generated in this study. Data shown are representative of 3 independent experiments. For blot source data, see Supplementary Fig.
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