SARS-CoV-2 infection of lung endothelial cells and pulmonary capillary microthrombosis uniklinik_hd SARSCoV2 COVID19 PulmonaryCapillaryMicrothrombosis LungEndothelialCells
By Tarun Sai LomteJul 4 2022Reviewed by Aimee Molineux In a recent study published in Pathology – Research and Practice, researchers evaluated the presence of RNA of severe acute respiratory syndrome coronavirus 2 in the lung tissue of deceased patients.
About the study In the current study, researchers examined the presence of SARS-CoV-2 RNA in deceased COVID-19 patients using RNA in situ hybridization . Forty COVID-19 patients were identified based on the interval between COVID-19 testing and death. Twelve patients were infected with SARS-CoV-2 variants of concern , such as Alpha , Beta , and Omicron variants, and the remaining were infected with ancestral SARS-CoV-2.
Findings The mean time from symptom onset to death was 10 days, and the median age at the time of death was 79 years. On average, patients were hospitalized for seven days. Histological examination revealed diffuse alveolar damage and edema in the exudative phase. The proliferative phase featured pneumocyte hyperplasia, squamous metaplasia, and desquamation, and subsequent development of organizing pneumonia leading to interstitial fibrosis.
The exudative phase of COVID-19 pneumonia was noted in 18 patients, the proliferative phase in six patients, and interstitial fibrosis in 14 patients. The remaining two patients could be categorized due to overlapping bronchopneumonia. The researchers observed an inverse association between the COVID-19 pneumonia stage and signal abundance. As such, a low viral load was detected in the fibrosis phase of COVID-19 pneumonia than in the exudative or proliferative phase.
Australia Latest News, Australia Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
SARS-CoV-2 productively infects human brain microvascular endothelial cells - Journal of NeuroinflammationBackground The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood–brain barrier (BBB). Methods RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes. Results A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins. Conclusions Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection.
Read more »
Impact of SARS-CoV-2 infection on menstrual cycleImpact of SARS-CoV-2 infection on menstrual cycle SARSCoV2 COVID19 MenstrualCycle MenstrualIrregularities
Read more »
Brain immune response to SARS-CoV-2 infectionIn a recently published article in the journal Current Opinion in Neurobiology, scientists at Washington University School of Medicine, St. Louis have described how immunological alterations associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection may impact brain activities and induce acute and post-acute neurological symptoms in coronavirus disease 2019 (COVID-19) patients.
Read more »
Multiplex Fragment Analysis for Flexible Detection of All SARS-CoV-2 Variants of ConcernAbstractBackground. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants continue to emerge, and effective tracking requires rapid return of re
Read more »
SARS-CoV-2 productively infects human brain microvascular endothelial cells - Journal of NeuroinflammationBackground The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood–brain barrier (BBB). Methods RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes. Results A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins. Conclusions Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection.
Read more »
Impact of SARS-CoV-2 infection on menstrual cycleImpact of SARS-CoV-2 infection on menstrual cycle SARSCoV2 COVID19 MenstrualCycle MenstrualIrregularities
Read more »