Researchers develop CRISPR-human organoids-single-cell RNA sequencing system to identify developmental brain defects in autism.
By Pooja Toshniwal PahariaSep 18 2023Reviewed by Benedette Cuffari, M.Sc. In a recent study published in Nature, researchers develop the clustered regularly interspaced short palindromic repeats -human organoids-single-cell ribonucleic acid sequencing system to identify developmental brain defects in autism.
About the study In the present study, researchers describe the CHOOSE system, which incorporates parallel inherited changes in single-cell transcriptomic display within mosaic brain organoids to identify dysfunctional traits for high-risk autism spectrum disorders genes throughout different kinds of cells in early brain developmental stages in cerebral organoids.
Using Simons Foundation Autism Research Initiative gene scores, the researchers investigated loss-of-function symptoms for 36 transcriptional regulatory genes with high ASD causal probability. All experiments were performed using human embryonic stem cells expressing eCas9. Flow cytometry-based guide RNA tests were used to measure the editing efficiencies of single-guide RNA pairs.
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