Why arteriosclerosis looks like an autoimmune disease unileidennews
. The study was approved by the Medical Ethics Committee of the UMCU . All blood samples were collected by venipuncture before surgery. Atherosclerosis specimens were obtained from primary CEAs, and estenotic plaques were excluded due to their different plaque composition as compared to primary atherosclerotic plaquesPeripheral venous blood was collected in K2-EDTA blood tubes . For scTCR-seq, blood was processed within 10 minutes after withdrawal .
Single-cell suspensions from blood and plaque from cohort 1 were stained with a mixture of extracellular antibodies for 30 minutes at 37 °C . All measurements were performed on a CytoFLEX S and analysed with FlowJo version 10.7 . A Shapiro log-normality test was performed, and a two-tailed Mann–Whitney test was performed using GraphPad analysis software to determine significance.PBMCs of cohort 2 were stained with TotalSeq-C antibodies against CD3, CD4, CD8 and CD14 .
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