Discovery of antibody structure could lead to treatment for Crimean Congo Hemorrhagic Fever virus UCRiverside NatureComms
An Octet R8 BLI protein analysis system was used to determine binding kinetics of GP38 mAb. Anti-hIgG Fc Capture sensors from Sartorius which was dipped into baseline 1× Octet kinetics buffer for 60 s. Sensors then encountered a 1 µg/mL antibody solution of either c13G8 or CC5-17 for 150 s as a loading step, following loading, they were then dipped into a second baseline containing 1× Octet Kinetics buffer.
The mixes were incubated for 1 h at +37 C. Then, medium was removed from Vero-E6 cells growing in 96-wells, and quadruplicate wells were infected with 40 µl each at +37 °C, 5% CO2 with periodic shaking. After 1 h, the inocula were removed and the cells overlaid with MEM containing 1.25% carboxymethylcellulose , 4% HI-FBS, sodium pyruvate, and penicillin-streptomycin.
Australia Latest News, Australia Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
Bras thrown on stage and Fazer steps in to save Dappy during N-Dubz gig in LeedsN-Dubz fever is sweeping the nation and hit Leeds again last night - as the troublesome trio brought their reunion tour to First Direct Arena.
Read more »
Researchers use a comparative study to model RSV prevention in childrenUsing computational modeling, a new paper compares the benefit of maternal vaccines versus the administration of respiratory syncytial virus (RSV)-specific monoclonal antibody (mAb) therapy against RSV.
Read more »
Characterization of bat viromes reveals the co-infection, spillover, and emergence risk of several zoonotic virusesResearchers characterized the mammal-infecting viruses in 149 bats, representing six genera and 15 species, sampled from Yunnan province, China using an unbiased meta-transcriptomics approach.
Read more »
The frequency, magnitude, and longevity of natural antibody responses against nine P. vivax vaccine candidate proteinsA new study aimed to evaluate the magnitude, longevity, and prevalence of naturally acquired IgG antibody responses against 9 P. vivax vaccine candidate antigens in patients with acute vivax malaria and were followed up for 180 days.
Read more »
Nanoparticles displaying SARS-CoV-1 and SARS-CoV-2 spikes induce broad antibody responses in animal modelNanoparticles displaying SARS-CoV-1 and SARS-CoV-2 spikes induce broad antibody responses in animal model Antibody Nanoparticles SARS SARSCoV2 Coronavirus Disease COVID iScience_CP UW WeillCornell UvA_Amsterdam scrippsresearch
Read more »