Nature research paper: Structure of the human DICER–pre-miRNA complex in a dicing state
Stem recognition by dsRBD and RIIID. The C-terminal dsRBD of DICER shows a large conformational change to accommodate dsRNA in the catalytic valley. Near the cleavage sites, this major groove of the RNA helix is expanded and sandwiched between dsRBD and RIIIDa. The mismatch of the GYM motif is recognized by R1855 of dsRBD.PAZ helix reorients to accommodate RNA in a dicing state. In a closed conformation, the PAZ helix sterically prevents the substrate from accessing the RNase III domains.
Model of pre-miRNA processing cycle by hDICER. Pre-miRNA binds to DICER, initially in a closed conformation where the helicase domain, the dsRBD and the PAZ helix sterically prevent the substrate from accessing the RNase III domains. From this closed form, DICER undergoes a large conformational change in the C-terminal dsRBD, the PAZ helix, and the helicase domain to allow the substrate to bind to the RNase III domains for cleavage.
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