Researchers uncover new insights into why individuals are affected differently by COVID-19 infection
: Fig. S2). For each miRNA, individuals who were outliers for miRNA expression—having miRNAs expression values more/less than + 3/-3 standard deviations from the mean—were removed.The expression of five miRNAs were validated using quantitative PCR . Total RNA was extracted from 92 samples , which were the same as those used for miRNA-sequencing, and re-quantified using the Qubit RNA Broad Range Kit . Of those, 88 samples passed initial quality control.
Viral load was quantified in saliva samples from 161 COVID-19 patients using a microfluidic ultra-sensitive quantitative test []. Per CDC recommendations, two assays were used for SARS-CoV-2 detection: 2019-nCoV_N1 and 2019-nCoV-N2 . The human RNase P assay was used as a control for RNA extraction and in RT-qPCR reactions. Each sample was analyzed using 9 replicates for N1, 9 replicates for N2, and 6 replicates for RP assays.
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