Tumors form temporary structures to avoid immunotherapy treatments elife elife
). Recipient mice were sub-lethally irradiated at a single dose of 600 rad, and injected i.v. twice, 3 days apart, with 1 × 10Cell linesB16F10 cells and 4T1 cells were purchased directly from ATCC in January 2017 and used no later than passage four. A375 and MDA-MB-231 cell lines were a kind gift from Professor Tamar Geiger from Tel Aviv University.
For NK isolation, splenic mononuclear cells were incubated with anti-CD4, anti-CD8, anti-B220, anti-CD19 and anti-CD115 magnetic beads according to the manufacturer’s instruction, and collected through negative selection.
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