Different celltypes in the brain are affected by tick infection, shows study NatureComms
mice in more detail and to provide a metric for viral infection in distinct brain regions, a Volume of Interest -based analysis was performed using VOI analysis in the PMOD VIEW tool and the OCUM atlas, resulting in 336 automatically delineated brain VOIs.
Statistical analysis was performed using an unpaired two-tailed t-test with Welch´s correction in GraphPad Prism. The level of significance was set atChP from the brain of LGTV-infectedmice were prepared for electron microscopy by cardiac perfusion with 20 mL of 0.1 M phosphate buffer, followed by 20 mL of 2.5% w/v glutaraldehyde and 4% w/v PFA in 0.1 M phosphate fixative solution.
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