SARS-CoV- 2 IgA and IgG antibodies are present in the breastmilk of women who have recovered from COVID-19 and last up to 8 months post-infection, according to a study published in BMCPregnancyChildbirth.
]. However, research on immunoglobulins in human milk derived from women affected by COVID-19 during pregnancy are mostly limited due to small group size or insufficient information about milk collection and its analysis. The purpose of this study was to investigate the concentrations of SARS-CoV-2 IgG and IgA in human milk and serum and factors that may affect their levels.
The concentration of breastmilk anti-SARS-CoV-2 IgG were measured using semi-quantitative enzyme linked immunosorbent assay and quantitative enzyme linked immunosorbent assay . Serum samples were diluted 101 times as suggested by the manufacturer, while breastmilk samples were diluted 5 times. The breastmilk samples were vortexed for 3 minutes immediately before sample dilution. The presence of serum and breastmilk anti-SARS-CoV-2 IgA was measured using semi-quantitative ELISA .
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