An article published in MicrobiomeJ presents mOTUs3: a software tool that enables accurate reference genome-independent species-level profiling of metagenomes by using universal marker gene sequences.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.Environment-specific membership of genomes in ref-, meta- and ext-mOTUs. A total of 499,512 genomes derived from 23 environments were used for the extension. The number of genomes was normalized by environments.
Tools with a lower score perform better, as the OPAL score is a sum over rank. The top plot represents the OPAL sum of scores, which is the sum over the four individual measures. mOTUs3 scored best in all categories, including the OPAL sum of scores.Taxonomic consistency of marker genes from the 499,512 genomes used to extend the mOTUs database. Marker genes from each genome were taxonomically annotated to evaluate taxonomic consistency.
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