Nanoprobes Could Help Decrease Nucleotide Detection Assay Cost lactoseintolerance nanoprobe nanoanalysis assay nucleotide dna
By Dr. Priyom Bose, Ph.D.Oct 12 2022Reviewed by Megan Craig, M.Sc. Gold nanoparticles have been commonly used in biomedical diagnostics because of their unique characteristic features, including high colloidal stability, prominent visible light absorption and scattering, a large surface area to volume ratio, and easy functionalization.
Adult-type hypolactasia or lactose intolerance is a condition where an individual cannot digest significant amounts of lactose. This inability is genetically linked and is commonly found in the European population. Detection of SNP Using Au-Nanoprobes AuNP has been considered a promising candidate for novel colorimetric assay to study biological samples, such as nucleic acids and proteins, due to their higher extinction coefficients for the plasmon band, which promotes the production of intense color.
Several procedures have been developed to optimize the performance of similar AuNPs-based assays. For instance, the utilization of a spacer between the nucleotide and the thiol group has been recommended because it can prevent steric hindrance upon target hybridization. Although this approach improved DNA loading, it reduced the interaction between DNA bases and Au.
Salt aging and the pH methods were used to functionalize 20 and 35 nanometer AuNPs with different molar concentrations of the thiol-modified 20-mer oligonucleotide. The pH method was favored because it led to the production of stable nanoprobes at low oligonucleotide: AuNPs ratios. Based on various analyses, the optimal functionalized ratio of 20 and 35 nanometer Au-nanoprobe was determined to be 150 and 1300, respectively.
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